Journal: BMC Gastroenterology

Article Title: Fibroblast activation protein-α-expressing fibroblasts promote the progression of pancreatic ductal adenocarcinoma

doi: 10.1186/s12876-015-0340-0

Figure Lengend Snippet: Effect of fibroblast activation protein-α (FAP) expressing NIH-3 T3 cells on invasiveness of MiaPaCa-2 cells. a Western blot analysis shows stable expression of FAP in NIH-3 T3 cells. b MiaPaCa-2 cells that were cocultured with NIH-3 T3 cells with/without FAP expression and then migrated through the polycarbonate membrane were stained with H&E, and counted in 4 randomly selected fields/well in 4 wells at x100 field magnification. The invasion assay was performed 13 times. *, P < 0.01

Article Snippet: MiaPaCa-2 and BxPC-3 pancreatic tumor cells and NIH-3 T3 fibroblasts were obtained from DS Pharma Biomedical (Osaka, Japan).

Techniques: Activation Assay, Expressing, Western Blot, Staining, Invasion Assay

Journal: BMC Gastroenterology

Article Title: Fibroblast activation protein-α-expressing fibroblasts promote the progression of pancreatic ductal adenocarcinoma

doi: 10.1186/s12876-015-0340-0

Figure Lengend Snippet: Cell cycle analysis of MiaPaCa-2 cells after coculture with NIH-3 T3 cells with/without FAP expression. a MiaPaCa-2 cells that were cocultured with NIH-3 T3 cells with/without FAP expression were examined for cell cycle distribution by using the integrated fluorescence intensity, which reflects cellular DNA content with the ImageXpress Micro Screening System. b Cell cycle distribution in MiaPaCa-2 cells that were cocultured with NIH 3 T3 cells with/without FAP. The experiments were repeated four times. Dark gray bars and bright gray bars indicate the G0/G1 phase fraction and S/G2/M phase fraction, respectively. *, P < 0.05

Article Snippet: MiaPaCa-2 and BxPC-3 pancreatic tumor cells and NIH-3 T3 fibroblasts were obtained from DS Pharma Biomedical (Osaka, Japan).

Techniques: Cell Cycle Assay, Expressing, Fluorescence

Journal: BMC Gastroenterology

Article Title: Fibroblast activation protein-α-expressing fibroblasts promote the progression of pancreatic ductal adenocarcinoma

doi: 10.1186/s12876-015-0340-0

Figure Lengend Snippet: Phosphorylation of Rb protein in MiaPaCa-2 cells that were cocultured with NIH-3 T3 cells with/without FAP. a Immunoblots for phosphorylated Rb (P-Rb) using MiaPaCa-2 cell lysates that were cocultured with NIH 3 T3 cells with/without FAP. b The P-Rb expression level was normalized to that of tubulin. The experiments were repeated three times. The black and gray bars indicate MiaPaCa-2 cells after coculture with FAP-expressing NIH-3 T3 cells and MiaPaCa-2 cells after coculture with NIH-3 T3 cells without FAP expression, respectively. *, P < 0.05

Article Snippet: MiaPaCa-2 and BxPC-3 pancreatic tumor cells and NIH-3 T3 fibroblasts were obtained from DS Pharma Biomedical (Osaka, Japan).

Techniques: Western Blot, Expressing

Journal: Gels

Article Title: Self-Assembling Peptide SCIBIOIII Hydrogel for Three-Dimensional Cell Culture That Promotes Wound Healing in Diabetic Mice

doi: 10.3390/gels9040265

Figure Lengend Snippet: Microscopic morphology of NIH3t3 at days 1, 3, and 5 in 2D and 3D cultures. The 2D group did not have peptide added, and the 3D group had 50 µL of SCIBIOIII peptide solution added in each well.

Article Snippet: High glucose DMEM medium with 10% FBS was used to cultivate NIH3t3 cells (Sangon Biotech, Shanghai, China) in a 37 °C incubator with 5% CO 2 .

Techniques:

Journal: Gels

Article Title: Self-Assembling Peptide SCIBIOIII Hydrogel for Three-Dimensional Cell Culture That Promotes Wound Healing in Diabetic Mice

doi: 10.3390/gels9040265

Figure Lengend Snippet: Cytotoxicity analysis of NIH3t3 in SCIBIOIII hydrogels. Calcein-AM staining analysis of NIH3t3 cells cultured in 2D and 3D on days 1, 3, and 5. Live cells are green.

Article Snippet: High glucose DMEM medium with 10% FBS was used to cultivate NIH3t3 cells (Sangon Biotech, Shanghai, China) in a 37 °C incubator with 5% CO 2 .

Techniques: Staining, Cell Culture